Applied Biosystems StepOnePlus Real-Time PCR System - PASSED Self Test - 4376592

Description

Applied Biosystems StepOnePlus Real-Time PCR and laptop with StepOne version 2.3 software, pulled from Thermo Fisher lab. StepOne 2.3 provides instrument control, experiment design, data collection, and data analysis capabilities, and also includes capabilities to collect melt curve data for High Resolution Melt (HRM) applications and the option to export in RDML for compatibility with MIQE guidelines.
The system passes all optic and cycle test diagnostics, and experiments were designed, sent to the instrument and run with no errors. All menus were navigated and all functions are accessible and respond correctly. The touchscreen works perfectly and the display is bright with no issues. The system also includes the cable to connect the instrument to the computer. I did not calibrate the instrument, and you will need to do calibrations runs for your dyes before using the system.
Manuals, and additional software is available on the Thermo Fisher website. More photos are available. Please ask if you have any questions.
Part Number: 4376592
System Software Version 2.2.3
Firmware Version 2.2.0.1 : 32849/44 : 3.4.12A
Dell Laptop with Windows 10 Pro, network cable, and power supply.
The StepOnePlus System supports many Real-Time PCR applications, including the following:
• SNP Genotyping
• Gene Expression Analysis
• MicroRNA Expression
• Protein Expression
• Translocation Analysis
• Gene Detection
• Viral Load Analysis
The included StepOne Software supports a variety of analysis methods, including the following:
• Standard Curve (absolute quantitation)
• Relat
ive Standard Curve
• Comparative Ct (ΔΔCt) (relative quantitation)
• Genotyping and Presence/Absence
• Melt Curve Analysis (as a standalone application)
• High Resolution Melting (as a standalone application)
Specifications:
Calibrated Dye: VIC, SYBR Green I, ROX, JOE, NED, FAM, TAMRA
Detection Method: SYBR, Primer-Probe Detection
Dynamic Range: Linear Dynamic Range greater than 9 log units (detection)
High-throughput Compatibility: Multiplexing
Passive Reference Dye: ROX (Separate Tube), No ROX, ROX (Pre-mixed)
Run Time: <2 hrs⁄run (Standard Mode), <40 min⁄run (Fast Mode)
Sensitivity: 1 copy
Temperature Range (Metric): 4-100°C
Thermal Cycling System: Peltier-Based System
Weight: 24 kg (53 lbs.)
Capacity: 96 x 0.1 ml tubes, 12 x 8-tube strips, 1 x 96-well plate
Display Type: LCD Touch Screen
Reaction Speed: Fast, Standard
Format: 96-well plate, 8-tube strips, 0.1 ml tubes
Optics: 4 emission filters, Blue LED excitation source, photodiode
Peak Block Ramp Rate: 4.6°C⁄sec
Reaction Volume Range: 10-30 µl (Standard curve experiments: 40 µl in standard mode is validated)
Sample Ramp Rate: Fast mode: ± 2.2°C⁄sec, Standard mode: ± 1.6°C⁄sec
Temperature Accuracy: 0.25°C (35 to 95°C) of display temperature
Temperature Uniformity: 0.25°C (35 to 95°C) of setpoint/display temperature
Dimensions: 24.6 cm/9.7 in.(W) x 42.7 cm/16.8 in.(D) x 51.2 cm/20.2 in.(H)